L-Carnitine Assay Kit (ab83392)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
Overview
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Product name
L-Carnitine Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Range
1 µM - 200 µM -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
Visit our FAQs page for tips and troubleshooting. -
Notes
Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Carnitine Assay Buffer WM 1 x 25ml Carnitine Converting Enzyme (lyophilized) Purple 1 vial Carnitine Development Mix (lyophilized) Green 1 vial Carnitine Probe (in DMSO) Red 1 x 200µl Carnitine Standard (10 µmol) (lyophilized) Yellow 1 vial Carnitine Substrate Mix Blue 1 x 400µl -
Research areas
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Relevance
Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L Carnitine is often sold as an antioxidant in nutritional supplements. Carnitine exists in two stereoisomers. Only L carnitine is biologically active. -
Alternative names
- Carnitine
- L Carnitine
Images
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Functional assay - 83392 L-Carnitine Assay Kit
Fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted.
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Functional assay - 83392 L-Carnitine Assay KitL-Carnitine measured in biologicals showing concentration (micromolar).
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Functional Studies - L-Carnitine Assay Kit (ab83392)Example of L-Carnitine standard curve obtained using ab83392.