Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)
![Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)](https://www.abcam.com/ps/products/247/ab247923/Images/ab247923-408160-anti-fntb-antibody-epr4706-bsa-and-azide-free-western-blot.jpg "Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4706] to FNTB - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-FNTB antibody [EPR4706] - BSA and Azide free
See all FNTB primary antibodies -
Description
Rabbit monoclonal [EPR4706] to FNTB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247923 is the carrier-free version of ab109748 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247923 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4706 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified) + C6 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?This data was developed using ab109748, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)](https://www.abcam.com/ps/products/247/ab247923/Images/ab247923-406184-anti-fntb-antibody-epr4706-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/5000 dilution (purified)
Lane 1 : JAR whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?This data was developed using ab109748, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)](https://www.abcam.com/ps/products/247/ab247923/Images/ab247923-2-anti-fntb-antibody-epr4706-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)This data was developed using ab109748, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling FNTB with purified ab109748 at 1/500. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000). -
![Western blot - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)](https://www.abcam.com/ps/products/247/ab247923/Images/ab247923-398944-anti-fntb-antibody-epr4706-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)All lanes : Anti-FNTB antibody [EPR4706] (ab109748) at 1/1000 dilution (unpurified)
Lane 1 : JAR cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDaThis data was developed using ab109748, the same antibody clone in a different buffer formulation.
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![Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)](https://www.abcam.com/ps/products/247/ab247923/Images/ab247923-5-benefits-of-recombinant-antibodies.png)
Anti-FNTB antibody [EPR4706] - BSA and Azide free (ab247923)