Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (ab83374)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.2 nmol/well
Overview
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Product name
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Sensitivity
> 0.2 nmol/well -
Assay time
0h 40m -
Product overview
Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.
The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).
The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
BCAA assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests BCAA Assay Buffer WM 1 x 25ml BCAA Enzyme Mix (lyophilized) Green 1 vial Leu Standard (1 µmol) Yellow 1 x 100µl WST Substrate Mix (lyophilized) Red 1 vial -
Research areas
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Relevance
The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects. -
Alternative names
- BCAA
- Isoleucine
- Leucine
- Valine
Images
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Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)
BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)Leucine Standard Curve performed according to the attached protocol.