Anti-PYCR2 antibody (ab103535)
Key features and details
- Rabbit polyclonal to PYCR2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PYCR2 antibody
See all PYCR2 primary antibodies -
Description
Rabbit polyclonal to PYCR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).
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Positive control
- HeLa cell lysate; PYCR2 transfected 293T cell line lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PYCR2 antibody (ab103535) This image is courtesy of an anonymous abreview.All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate- 18µg
Lane 2 : HeLa whole cell lysate- 36µg
Lane 3 : RPE-1 whole cell lysate- 18µg
Lane 4 : RPE-1 whole cell lysate- 36µg
Secondary
All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutesBlocking: 10% Milk
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Western blot - Anti-PYCR2 antibody (ab103535)Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg
Predicted band size: 34 kDa
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Western blot - Anti-PYCR2 antibody (ab103535)All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution
Lane 1 : PYCR2 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
