Anti-HSD3B2 antibody (ab154385)
Key features and details
- Rabbit polyclonal to HSD3B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-HSD3B2 antibody
See all HSD3B2 primary antibodies -
Description
Rabbit polyclonal to HSD3B2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 126-372 of Human HSD3B2 (UniProt: P26439).
-
Positive control
- Raji and HeLaS3 whole cell lysates; HeLa cells; Human A549 xenograft tissue.
-
General notes
HSD3B2 and HSD3B1 proteins are quite similar in sequence so this product may react with both proteins.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling HSD3B2 with ab154385 at 1/100 dilution.
-
All lanes : Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : HeLaS3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
-
ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.