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Signal Transduction Growth Factors/Hormones Hormones

Anti-HSD3B2 antibody (ab154385)

Price and availability

301 536 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-HSD3B2 antibody (ab154385)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HSD3B2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HSD3B2 antibody
    See all HSD3B2 primary antibodies
  • Description

    Rabbit polyclonal to HSD3B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 126-372 of Human HSD3B2 (UniProt: P26439).

  • Positive control

    • Raji and HeLaS3 whole cell lysates; HeLa cells; Human A549 xenograft tissue.
  • General notes

    HSD3B2 and HSD3B1 proteins are quite similar in sequence so this product may react with both proteins.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Developmental Biology
    • Reproduction
    • Hormones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385)
    Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling HSD3B2 with ab154385 at 1/100 dilution.
  • Western blot - Anti-HSD3B2 antibody (ab154385)
    Western blot - Anti-HSD3B2 antibody (ab154385)
    All lanes : Anti-HSD3B2 antibody (ab154385) at 1/1000 dilution

    Lane 1 : Raji whole cell lysate
    Lane 2 : HeLaS3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-HSD3B2 antibody (ab154385)
    Immunocytochemistry/ Immunofluorescence - Anti-HSD3B2 antibody (ab154385)
    Immunofluorescent analysis of methanol-fixed HeLa cells, labeling HSD3B2 with ab154385 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSD3B2 antibody (ab154385) This image is courtesy of an Abreview submitted by Hilde Nelis

    ab154385 staining HSD3B2 in horse cumulus cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% bovine serum for 1 hour at 18°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 2% BSA) for 18 hours at 18°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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