Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)
![Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)](https://www.abcam.com/ps/products/75/ab75710/Images/ab75710-90736-anti-hsd3b2-antibody-373ct913-western-blot.jpg "Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)")
Key features and details
- Mouse monoclonal [373CT9.1.3] to HSD3B2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-HSD3B2 antibody [373CT9.1.3]
See all HSD3B2 primary antibodies -
Description
Mouse monoclonal [373CT9.1.3] to HSD3B2 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Cynomolgus monkey, Macaque monkey, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human HSD3B2 aa 1-100 conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in human adrenal tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
373CT9.1.3 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Human adrenal normal tissue lysate - total protein (ab29249) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Blocked using 3% milk. -
![Flow Cytometry - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)](https://www.abcam.com/ps/products/75/ab75710/Images/ab75710-208157-anti-hsd3b2-antibody-373ct913-flow-cytometry.jpg)
Flow Cytometry - Anti-HSD3B2 antibody [373CT9.1.3] (ab75710)Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
