Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)
Key features and details
- Mouse monoclonal to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Mouse monoclonal to Indoleamine 2, 3-dioxygenase -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-404 of Human Indoleamine 2, 3-dioxygenase
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General notes
This product was changed from ascites to tissue culture supernatant on 30th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)
Indoleamine 2 3-dioxygenase antibody (ab55305) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Indoleamine 2, 3-dioxygenase antibody (ab55305)Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
