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Signal Transduction Metabolism Amino Acids

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]
    See all Indoleamine 2, 3-dioxygenase primary antibodies
  • Description

    Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Indoleamine 2, 3-dioxygenase aa 100-200.

  • Positive control

    • WB: HeLa treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate,
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1230Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
    Lane 1 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
    Lane 2 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate at 15 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa



    The expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.

  • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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