Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)
![Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)](https://www.abcam.com/ps/products/229/ab229377/Images/ab229377-295278-anti-vegfa-antibody-epr20705-western-blot.jpg "Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20705] to VEGFA - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VEGFA antibody [EPR20705] - BSA and Azide free
See all VEGFA primary antibodies -
Description
Rabbit monoclonal [EPR20705] to VEGFA - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal vessel lysate; HeLa, HUVEC, SH-SY5Y, Neuro-2a, bEND.3, C6 and RAW 264.7 whole cell lysates.
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General notes
Ab229377 is the carrier-free version of ab214424. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab229377 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20705 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)All lanes : Anti-VEGFA antibody [EPR20705] (ab214424) at 1/1000 dilution
Lane 1 : Human fetal vessel lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 6 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 7 : C6 (rat glial tumor cell line) whole cell lysate
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST
Exposure time : Lanes 1-4/7-8: 3 minutes; Lane 5: 41 seconds; Lane 6: 15 seconds.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214424).
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![Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)](https://www.abcam.com/ps/products/229/ab229377/Images/ab229377-2-benefits-of-recombinant-antibodies.png)
Anti-VEGFA antibody [EPR20705] - BSA and Azide free (ab229377)
