Anti-VEGFA antibody [EPR20705] (ab214424)
![Anti-VEGFA antibody [EPR20705] (ab214424)](https://www.abcam.com/ps/products/214/ab214424/Images/ab214424-295234-anti-vegfa-antibody-epr20705-western-blot.jpg "Anti-VEGFA antibody [EPR20705] (ab214424)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20705] to VEGFA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VEGFA antibody [EPR20705]
See all VEGFA primary antibodies -
Description
Rabbit monoclonal [EPR20705] to VEGFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal vessel lysate; HeLa, HUVEC, SH-SY5Y, Neuro-2a, bEND.3, C6 and RAW 264.7 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20705 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VEGFA antibody [EPR20705] (ab214424)All lanes : Anti-VEGFA antibody [EPR20705] (ab214424) at 1/1000 dilution
Lane 1 : Human fetal vessel lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 6 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 7 : C6 (rat glial tumor cell line) whole cell lysate
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST
Exposure time : Lanes 1-4/7-8: 3 minutes; Lane 5: 41 seconds; Lane 6: 15 seconds.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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![Anti-VEGFA antibody [EPR20705] (ab214424)](https://www.abcam.com/ps/products/214/ab214424/Images/ab214424-2-benefits-of-recombinant-antibodies.png)
Anti-VEGFA antibody [EPR20705] (ab214424)
