Anti-VEGFA antibody (ab9570)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VEGFA antibody
See all VEGFA primary antibodies -
Description
Rabbit polyclonal to VEGFA -
Host species
Rabbit -
Specificity
Reactive with VEGF 121 as well as VEGF 165. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human VEGFA aa
27-191
.
Sequence:27-191
Database link: P15692-4 -
Positive control
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1316.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-VEGFA antibody (ab9570)Lanes 2-12 : Anti-VEGFA antibody (ab9570) at 0.2 µg/ml
Lane 1 : MultiMark MultiColor Standard
Lane 2 : Recombinant Human VEGF at 0.25 µg
Lane 3 : Recombinant Human VEGF at 0.125 µg
Lane 4 : Recombinant Human VEGF at 0.0625 µg
Lane 5 : Recombinant Human VEGF at 0.03125 µg
Lane 6 : Recombinant Human VEGF at 0.015625 µg
Lane 7 : Recombinant Human VEGF at 0.0078 µg
Lane 8 : Recombinant Human VEGF at 0.0039 µg
Lane 9 : Recombinant Human VEGF at 0.00195 µg
Lane 10 : Recombinant Human VEGF at 0.000975 µg
Lane 11 : Recombinant Human VEGF at 0.0004875 µg
Lane 12 : Recombinant Human VEGF at 0.00024 µg
Performed under non-reducing conditions.
Observed band size: 38.2 kDa why is the actual band size different from the predicted?
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Sandwich ELISA - Anti-VEGFA antibody (ab9570)Detected human VEGF in a sandwich ELISA within the range 16-1000 pg/ml
- 0.5 µg/ml of capture antibody (ab9570)
- 0.25 µg/ml of detection antibody (ab83132) -
Western blot - Anti-VEGFA antibody (ab9570)Lanes 2-12 : Anti-VEGFA antibody (ab9570)
Lane 1 : Molecular weight ladder
Lane 2 : Recombinant human VEGFA at 0.25 µg
Lane 3 : Recombinant human VEGFA at 0.125 µg
Lane 4 : Recombinant human VEGFA at 0.0625 µg
Lane 5 : Recombinant human VEGFA at 0.03125 µg
Lane 6 : Recombinant human VEGFA at 0.015625 µg
Lane 7 : Recombinant human VEGFA at 0.0078 µg
Lane 8 : Recombinant human VEGFA at 0.0039 µg
Lane 9 : Recombinant human VEGFA at 0.00195 µg
Lane 10 : Recombinant human VEGFA at 0.000975 µg
Lane 11 : Recombinant human VEGFA at 0.0004875 µg
Lane 12 : Recombinant human VEGFA at 0.00024 µg
Performed under reducing conditions.
