Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VEGFA antibody [EPR21219] (ab213244)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [EPR21219] to VEGFA
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-VEGFA antibody [EPR21219]
    See all VEGFA primary antibodies

  • Description

    Human monoclonal [EPR21219] to VEGFA

  • Host species

    Human

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human VEGFA aa 1-200.
    Database link: P15692-4

  • Positive control

    • IHC-P: FFPE human normal kidney tissue sections.

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody.  
    Human monoclonal antibody.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EPR21219] (ab213244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [EPR21219] (ab213244)

    IHC image of VEGFA staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab213244, 10ug/ml, for 15 mins at room temperature. An HRP-conjugated goat anti-Human IgG secondary was used for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-VEGFA antibody [EPR21219] (ab213244)
    Anti-VEGFA antibody [EPR21219] (ab213244)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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