Anti-VEGFA antibody (ab46154)
Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: WB, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VEGFA antibody
See all VEGFA primary antibodies -
Description
Rabbit polyclonal to VEGFA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
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Positive control
- Purchase matching WB positive control:Recombinant Human VEGFA protein
- ab46154 gave a positive result in the following whole cell lysates: Recombinant Human VEGFA protein Recombinant Mouse VEGFA protein HCT116 whole cell lysate
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General notes
New batches of this antibody are no longer guaranteed in ICC/IF, IHC-P or IHC-Fr as they have not passed our stringent batch testing criteria. Please contact customer support for any specific queries. We recommend ab52917 as an alternative for ICC/IF and IHC-P.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody (ab46154) Courtesy of Feng Y et al. Sci Rep. 2017; 7: 44810. doi: 10.1038/srep44810 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
Immunohistochemical analysis of adult proestrous mouse ovarian follicles staining CYP19/aromatase with ab35604, and VEGFA with ab46154. Staining of follicles at different stages using specific markers (upper panels) together with histological pictures using hematoxylin and eosin staining (lower panels).
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Western blot - Anti-VEGFA antibody (ab46154)Anti-VEGFA antibody (ab46154) at 1 µg/ml + HCT116 whole cell lysate at 20 µg
Secondary
Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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ELISA - Anti-VEGFA antibody (ab46154)Ab46154 was tested using indirect ELISA. The wells were coated with peptide (1 µg x mL-1 at 100 µL per well) overnight at 4°C, followed by a 1% fat-free milk blocking step for 1 hour at room temperature. The primary antibody (ab46154) was added at a range of dilutions (50 µL per well) for 1 hour at room temperature. Ab97080 (Goat Anti-Rabbit IgG H&L-HRP) was used as a secondary antibody at 1:50.000 dilution for 1 hour at room temperature and signal was developed with TMB substrate.
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Western blot - Anti-VEGFA antibody (ab46154)All lanes : Anti-VEGFA antibody (ab46154) at 1 µg/ml
Lane 1 : Recombinant Human VEGFA protein (His tag) (ab204773)
Lane 2 :Recombinant mouse VEGFA protein (Active) (ab185265)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
