Mouse GAS6 ELISA Kit (ab278087)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.4 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse GAS6 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3.4% Inter-assay Sample n Mean SD CV% Plasma 3 5% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.4 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 100% - 118% Serum 108 92% - 118% Cell culture media 89 87% - 90% Hep Plasma 83 67% - 99% EDTA Plasma 108 103% - 116% Cit plasma 105 94% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GAS6 ELISA kit (ab278087) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse GAS6 protein in mouse serum, plasma and cell culture supernatant.. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GAS6 with 8.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Gas6 (Growth arrest-specific protein 6), also known as AXL receptor tyrosine kinase ligand, is a secreted ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. Gas6 signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse GAS6 Capture Antibody 1 x 600µl 10X Mouse GAS6 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse GAS6 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. -
Tissue specificity
Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
Post-translational
modificationsIsoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AXL receptor tyrosine kinase ligand
- AXL stimulatory factor
- AXLLG
see all -
Database links
- Entrez Gene: 14456 Mouse
- SwissProt: Q61592 Mouse
- Unigene: 3982 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse GAS6 standard curve in Sample Diluent NS.The GAS6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GAS6 in mouse serum, plasma and kidney supernatant samples.The concentrations of GAS6 were measured in duplicates, interpolated from the GAS6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 5%, plasma (heparin) 10% and kidney supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GAS6 concentration was determined to be 45.4 ng/mL in neat serum, 44.3 ng/mL in neat plasma (citrate), 71.8 ng/mL in neat plasma (EDTA), 49.3 ng/mL in neat plasma (heparin) and 2.0 ng/mL in neat kidney supernatant.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
