Anti-Ret (phospho Y1062) antibody (ab51103)
Key features and details
- Rabbit polyclonal to Ret (phospho Y1062)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ret (phospho Y1062) antibody
See all Ret primary antibodies -
Description
Rabbit polyclonal to Ret (phospho Y1062) -
Host species
Rabbit -
Specificity
ab51103 detects endogenous levels of Ret only when phosphorylated at tyrosine 1062. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthetic phospho-peptide derived from human Ret around the phosphorylation site of tyrosine 1062 (K-L-YP-G-M).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51103 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ret (phospho Y1062) antibody (ab51103)All lanes : Anti-Ret (phospho Y1062) antibody (ab51103) at 1/500 dilution
Lane 1 : K562 cell lysate.
Lane 2 : K562 cell Lysate. For this panel, ab51103 was pre-incubated with immunizing peptide.
Predicted band size: 124 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret (phospho Y1062) antibody (ab51103)Ab51103 staining human normal testis tissue. Staining is localised to cell membranes.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
