Anti-Ezrin antibody [6F1C3] (ab204954)
Key features and details
- Mouse monoclonal [6F1C3] to Ezrin
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Ezrin antibody [6F1C3]
See all Ezrin primary antibodies -
Description
Mouse monoclonal [6F1C3] to Ezrin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human Ezrin aa 250-500. Expressed in E coli.
Database link: P15311 -
Positive control
- Recombinant Ezrin; Ezrin transfected HEK293 lysate; Jurkat, A431, MCF-7 and HeLa whole cell lysate (ab150035); HeLa cells, A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6F1C3 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 69 kDa
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Flow cytometric analysis of A431 cells labeling Ezrin with ab204954 at 1/200 (green) or negative control (red)
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Immunofluorescent analysis of Hela cells labeling Ezrin with ab204954 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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All lanes : Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 69 kDa
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Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution + Human Ezrin (aa: 292-464) recombinant protein.
Predicted band size: 69 kDa(Expected MW is 47.1 kDa)