Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD105 antibody [EPR19911] (ab206419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19911] to CD105
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD105 antibody [EPR19911]
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR19911] to CD105

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 and HUVEC whole cell lysates; human fetal kidney lysate. IHC-P: Human tonsil and kidney cancer tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
    Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
    All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : CD105  knockout HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 70 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab206419 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab206419 was shown to recognize CD105  in wild-type HeLa cells as signal was lost at the expected MW in CD105  knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab206419 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
    Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
    All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution

    Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 70 kDa
    Observed band size: 90,95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1692830, PMID: 18223685, PMID: 11164421).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm on human tonsil vascular endothelium is observed [PMID:17502949].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)

    Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasm on human kidney vascular endothelium is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CD105 antibody [EPR19911] (ab206419)
    Anti-CD105 antibody [EPR19911] (ab206419)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD105 antibody [EPR19911] (ab206419)

© 2021 Astana Biomed Group LLP. All rights reserved.