Anti-CD105 antibody [EPR19911] (ab206419)
![Anti-CD105 antibody [EPR19911] (ab206419)](https://www.abcam.com/ps/products/206/ab206419/Images/ab206419-338203-anti-cd105-antibody-epr19911-western-blot.jpg "Anti-CD105 antibody [EPR19911] (ab206419)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19911] to CD105
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CD105 antibody [EPR19911]
See all CD105 primary antibodies -
Description
Rabbit monoclonal [EPR19911] to CD105 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 and HUVEC whole cell lysates; human fetal kidney lysate. IHC-P: Human tonsil and kidney cancer tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19911 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD105 antibody [EPR19911] (ab206419)All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaLanes 1 - 2: Merged signal (red and green). Green - ab206419 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab206419 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab206419 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD105 antibody [EPR19911] (ab206419)](https://www.abcam.com/ps/products/206/ab206419/Images/ab206419-263454-anti-cd105-antibody-epr19911-western-blot.jpg)
Western blot - Anti-CD105 antibody [EPR19911] (ab206419)All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 70 kDa
Observed band size: 90,95 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 1692830, PMID: 18223685, PMID: 11164421).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)](https://www.abcam.com/ps/products/206/ab206419/Images/ab206419-263452-anti-cd105-antibody-epr19911-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm on human tonsil vascular endothelium is observed [PMID:17502949].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)](https://www.abcam.com/ps/products/206/ab206419/Images/ab206419-263450-anti-cd105-antibody-epr19911-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm on human kidney vascular endothelium is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-CD105 antibody [EPR19911] (ab206419)](https://www.abcam.com/ps/products/206/ab206419/Images/ab206419-6-benefits-of-recombinant-antibodies.png)
Anti-CD105 antibody [EPR19911] (ab206419)
