Anti-CD105 antibody [3A9] (ab114052)
![Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126922-anti-cd105-antibody-3a9-flow-cytometry.jpg "Anti-CD105 antibody [3A9] (ab114052)")
Key features and details
- Mouse monoclonal [3A9] to CD105
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD105 antibody [3A9]
See all CD105 primary antibodies -
Description
Mouse monoclonal [3A9] to CD105 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Purified recombinant fragment of Human CD105, expressed in E. coli.
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Positive control
- IHC-P: Human kidney cancer and stomach cancer tissues ICC/IF: HepG2 cells WB: CD105-hIgGFc transfected HEK293 cell lysate
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR156609-14 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3A9 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD105 antibody [3A9] (ab114052)Flow cytometric analysis of CD105 in HepG2 cells, using ab114052 at a 1/200 dilution (green) and negative control (purple).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126926-anti-cd105-antibody-3a9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [3A9] (ab114052)ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human kidney cancer tissue by Immunohistochemistry, with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126925-anti-cd105-antibody-3a9-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [3A9] (ab114052)ab114052, at a 1/200 dilution, staining CD105 in paraffin embedded Human stomach cancer tissue by Immunohistochemistry, with DAB staining. -
![Western blot - Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126924-anti-cd105-antibody-3a9-western-blot.jpg)
Western blot - Anti-CD105 antibody [3A9] (ab114052)All lanes : Anti-CD105 antibody [3A9] (ab114052) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : CD105-hIgGFc transfected HEK293 cell lysate
Predicted band size: 71 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126923-anti-cd105-antibody-3a9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [3A9] (ab114052)ab114052, at a 1/200 dilution, staining CD105 in HepG2 cells by Immunofluorescence (green).
Blue: DRAQ5 fluorescent DNA dye.
Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![ELISA - Anti-CD105 antibody [3A9] (ab114052)](https://www.abcam.com/ps/products/114/ab114052/Images/ab114052-126921-anti-cd105-antibody-3a9-elisa.jpg)
ELISA - Anti-CD105 antibody [3A9] (ab114052)ELISA using ab114052.
