Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21846] to CD105 - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD105 antibody [EPR21846] - BSA and Azide free
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR21846] to CD105 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse E14.5 liver tissue.

  • General notes

    Ab231832 is the carrier-free version of ab221675. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Flow Cytometry - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line (left panel) and bEND.3 (mouse brain endothelioma cell line) cell line (right panel) labeling CD105 with ab221675 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on total viable cells.

    Negative control: NIH/3T3. (PMID: 8194490).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. Positive staining in the endothelial cells of blood vessels in mouse brain tissue section (PMID: 24699047). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Positive staining in the hepatic sinusoidal endothelial cells on mouse liver tissue section (PMID: 12947156; 24507660). The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunoprecipitation - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunoprecipitation - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    CD105 was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab221675 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221675 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: bEND.3 whole cell lysate 10 µg (Input). 

    Lane 2: ab221675 IP in bEND.3 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221675 in bEND.3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse lung is observed (PMID: 14528280). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD105 with ab221675 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Neuro-2a cell line. Negative control: NIH/3T3 (PMID: 8194490).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse E14.5 liver is observed (PMID: 18805961). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221675).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)
    Anti-CD105 antibody [EPR21846] - BSA and Azide free (ab231832)

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