Anti-CD146 antibody - C-terminal (ab228540)
Key features and details
- Rabbit polyclonal to CD146 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CD146 antibody - C-terminal
See all CD146 primary antibodies -
Description
Rabbit polyclonal to CD146 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CD146 (C terminal). The exact sequence is proprietary.
Database link: P43121 -
Positive control
- WB: Rat liver extract; HCT 116 whole cell lysate. IHC-P: J5 xenograft; Mouse skin and colon tissues. ICC/IF: HUVEC cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (ab228540)
Paraffin-embedded J5 xenograft stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD146 antibody - C-terminal (ab228540)4% paraformaldehyde-fixed HUVEC (human umbilical vein endothelial cell line) cells stained for CD146 (green) using ab228540 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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Western blot - Anti-CD146 antibody - C-terminal (ab228540)Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution + Rat liver extract at 50 µg
Predicted band size: 72 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-CD146 antibody - C-terminal (ab228540)Anti-CD146 antibody - C-terminal (ab228540) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 72 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (ab228540)Paraffin-embedded mouse skin tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD146 antibody - C-terminal (ab228540)Paraffin-embedded mouse colon tissue stained for CD146 using ab228540 at 1/500 dilution in immunohistochemical analysis.
