Anti-Hsp90 beta (phospho S254) antibody (ab51136)
Key features and details
- Rabbit polyclonal to Hsp90 beta (phospho S254)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp90 beta (phospho S254) antibody
See all Hsp90 beta primary antibodies -
Description
Rabbit polyclonal to Hsp90 beta (phospho S254) -
Host species
Rabbit -
Specificity
Detects endogenous levels of HSP90B only when phosphorylated at serine 254. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Hsp90 beta (phospho S254) antibody (ab51136)All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/500 dilution
Lane 1 : extracts from Hela cells treated with TNF-a with immunizing peptide
Lane 2 : extracts from Hela cells treated with TNF-a
Lysates/proteins at 0.02 µg/ml per lane.
Predicted band size: 83 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta (phospho S254) antibody (ab51136)Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.
