Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD105 antibody [MEM-229] (ab69772)

Key features and details

  • Mouse monoclonal [MEM-229] to CD105
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Human, Pig
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CD105 antibody [MEM-229]
    See all CD105 primary antibodies

  • Description

    Mouse monoclonal [MEM-229] to CD105

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, Pig

  • Immunogen

    Tissue, cells or virus corresponding to Human CD105.

  • Positive control

    • Kg1 human acute myelogenous leukemia cell line. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
    Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)

    ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
    Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
    Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5µg/mL. DAPI (blue) was used as nuclear counterstain. 

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