Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18008-67] to CD147 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, IHC-P, ICC, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD147 antibody [EPR18008-67] - BSA and Azide free
    See all CD147 primary antibodies

  • Description

    Rabbit monoclonal [EPR18008-67] to CD147 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251535 is the carrier-free version of ab212057.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Western blot - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Anti-CD147 antibody [EPR18008-67] (ab212057) at 1/5000 dilution + His-tagged mouse CD147 recombinant protein fragment (aa140-325) at 0.002 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab212057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Western blot - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    All lanes : Anti-CD147 antibody [EPR18008-67] (ab212057) at 1/5000 dilution

    Lane 3 : WEHI-3 (mouse leukemia cell line) whole cell lysate
    Lane 4 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa
    Observed band size: 45-55 kDa why is the actual band size different from the predicted?



    This data was developed using ab212057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 minutes; Lane 3: 10 seconds; Lane 4: 1 second.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16721788; 23966157).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    This data was developed using ab212057, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling CD147 with ab212057 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membranous staining on mouse intestine is observed. Counter stained withematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Immunocytochemistry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    This data was developed using ab212057, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelioma cell line) cells labeling CD147 with ab212057 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membranous staining on bEnd.3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative control are is as follows: -ve control 1: ab212057 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunocytochemistry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Immunocytochemistry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    This data was developed using ab212057, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse lymphoblast B cell lymphoma cell line) cells labeling CD147 with ab212057 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cell membranous staining on bEnd.3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative control are is as follows: -ve control 1: ab212057 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Flow Cytometry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Flow Cytometry - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    This data was developed using ab212057, the same antibody clone in a different buffer formulation.Flow cytometric analysis of fresh mouse thymocytes labeling CD147 with ab212057 at 1/200 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/500 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Immunoprecipitation - Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)

    This data was developed using ab212057, the same antibody clone in a different buffer formulation.

    CD147 was immunoprecipitated from 1 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab212057 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab212057 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: RAW 264.7 whole cell lysate 10µg (Input).

    Lane 2: ab212057 IP in RAW 264.7 whole cell lysate.

    Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab212057 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)
    Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD147 antibody [EPR18008-67] - BSA and Azide free (ab251535)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.