Anti-CD147 antibody [OX47] (ab238462)
![Anti-CD147 antibody [OX47] (ab238462)](https://www.abcam.com/ps/products/238/ab238462/Images/ab238462-355358-antiratCD147spleensplenocytes-Fow-cytometry.jpg "Anti-CD147 antibody [OX47] (ab238462)")
Key features and details
- Mouse monoclonal [OX47] to CD147
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD147 antibody [OX47]
See all CD147 primary antibodies -
Description
Mouse monoclonal [OX47] to CD147 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to CD147. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
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Positive control
- IHC-Fr: Rat spleen tissue. Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX47 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD147 antibody [OX47] (ab238462)
ab150117) was used at 1/2000 for 30 min on ice.
ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
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![Immunohistochemistry (Frozen sections) - Anti-CD147 antibody [OX47] (ab238462)](https://www.abcam.com/ps/products/238/ab238462/Images/ab238462-353162-anti-cd147-ox47-ihcfr-rat-colon.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD147 antibody [OX47] (ab238462)IHC image of CD147 staining in a section of frozen normal rat colon*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab238462 (shown in green) at 5g/ml.
The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
