Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTF1 antibody [EPR5955(2)] (ab133737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5955(2)] to TTF1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TTF1 antibody [EPR5955(2)]
    See all TTF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5955(2)] to TTF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human TTF1.

  • Positive control

    • HeLa, Human fetal lung, Rat lung and Mouse lung lysates; Human lung adenocarcinoma and Human papillary carcinoma of thyroid gland tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 9.50 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5955(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1 with ab133737 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Western blot - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    All lanes : Anti-TTF1 antibody [EPR5955(2)] (ab133737) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : Rat lung lysate
    Lane 4 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1 with ab133737 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing negative staining in human Normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing negative staining in human Glioma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing negative staining in human Breast carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing positive staining in human Lung adenocarcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR5955(2)] (ab133737)

    Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    OI-RD Scanning - Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TTF1 antibody [EPR5955(2)] (ab133737)
    Anti-TTF1 antibody [EPR5955(2)] (ab133737)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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