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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-KLF4 antibody (ab216968)

Anti-KLF4 antibody (ab216968)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KLF4
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human KLF4 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O43474

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon carcinoma lysate and tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody (ab216968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody (ab216968)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue, labeling KLF4 using ab216968 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Western blot - Anti-KLF4 antibody (ab216968)
    Western blot - Anti-KLF4 antibody (ab216968)
    All lanes : Anti-KLF4 antibody (ab216968) at 1/200 dilution

    Lane 1 : rat kidney lysate
    Lane 2 : Human colon carcinoma lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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