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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-KLF4 antibody (ab216875)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-KLF4 antibody (ab216875)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to KLF4
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87, C2C12, THP-1, J774A.1, PC-3, HT-29, A549 and HeLa whole cell lysates. ICC/IF: PC-3 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (ab216875)
    Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (ab216875)

    Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 µg/ml (green).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: C and D.

  • Western blot - Anti-KLF4 antibody (ab216875)
    Western blot - Anti-KLF4 antibody (ab216875)
    All lanes : Anti-KLF4 antibody (ab216875) at 1/1000 dilution

    Lanes 1 & 13 : MW ladder (opal pre-stained)
    Lane 2 : U-87 whole cell lysate
    Lane 3 : C2C12 whole cell lysate
    Lane 4 : NIH/3T3 whole cell lysate
    Lane 5 : THP-1 whole cell lysate
    Lane 6 : J774A.1 whole cell lysate
    Lane 7 : PC-3 whole cell lysate
    Lane 8 : HT-29 whole cell lysate
    Lane 9 : Mouse colon lysate
    Lane 10 : A549 whole cell lysate
    Lane 11 : MOLT-4 whole cell lysate
    Lane 12 : HeLa whole cell lysate

    Secondary
    All lanes : Rabbit secondary HRP antibody at 1/70000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking buffer: Blotto 5%.

    Dilution buffer: 1X PBS, 0.01% azide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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