Anti-KLF4 antibody (ab216875)
Key features and details
- Rabbit polyclonal to KLF4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-KLF4 antibody
See all KLF4 primary antibodies -
Description
Rabbit polyclonal to KLF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB MouseHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: U-87, C2C12, THP-1, J774A.1, PC-3, HT-29, A549 and HeLa whole cell lysates. ICC/IF: PC-3 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 µg/ml (green).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: C and D.
-
All lanes : Anti-KLF4 antibody (ab216875) at 1/1000 dilution
Lanes 1 & 13 : MW ladder (opal pre-stained)
Lane 2 : U-87 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : J774A.1 whole cell lysate
Lane 7 : PC-3 whole cell lysate
Lane 8 : HT-29 whole cell lysate
Lane 9 : Mouse colon lysate
Lane 10 : A549 whole cell lysate
Lane 11 : MOLT-4 whole cell lysate
Lane 12 : HeLa whole cell lysate
Secondary
All lanes : Rabbit secondary HRP antibody at 1/70000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: Blotto 5%.
Dilution buffer: 1X PBS, 0.01% azide.