Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3951] to SATB1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SATB1 antibody [EPR3951] - BSA and Azide free
    See all SATB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3951] to SATB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239944 is the carrier-free version of ab109122. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239944 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

    Ab109122 staining SATB1 in THP-1 (Human monocytic leukemia monocyte) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on THP-1 cell line. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

  • Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunocytochemistry/ Immunofluorescence - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

    Ab109122 staining SATB1 in Jurkat (Human T cell leukemia T lymphocyte) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on Jurkat cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944) This image is courtesy of an Abreview submitted by Ahmar Aziz.

    ab109122 staining SATB1 in mouse skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde, blocked with 10% goat serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 12 hours. A FITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

    ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human breast carcinoma, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

    ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human ovarian adenocarcinoma tissue, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

    ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109122).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)
    Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SATB1 antibody [EPR3951] - BSA and Azide free (ab239944)

© 2021 Astana Biomed Group LLP. All rights reserved.