Anti-SATB1 antibody - C-terminal (ab228772)
Key features and details
- Rabbit polyclonal to SATB1 - C-terminal
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SATB1 antibody - C-terminal
See all SATB1 primary antibodies -
Description
Rabbit polyclonal to SATB1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Rhesus monkey -
Immunogen
Recombinant fragment within Human SATB1 (C terminal). The exact sequence is proprietary.
Database link: Q01826 -
Positive control
- WB: Jurkat and THP-1 whole cell lysates; Mouse thymus tissue extract. IP: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue. ChIP: Jurkat chromatin extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-SATB1 antibody - C-terminal (ab228772) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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SATB1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab228772. Western blot was performed from the immunoprecipitate using ab228772 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.
Lane 1: 2 μg of preimmune rabbit IgG instead of ab228772 in HEK-293T whole cell lysate.
Lane 2: ab228772 IP in HEK-293T whole cell lysate. -
Paraffin-embedded human colon carcinoma tissue stained for SATB1 using ab228772 at 1/250 dilution in immunohistochemical analysis.
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Cross-linked ChIP was performed with Jurkat (human T cell leukemia cell line from peripheral blood) chromatin extract and 5 μg of either control rabbit IgG or ab228772. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
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Anti-SATB1 antibody - C-terminal (ab228772) at 1/500 dilution + THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Anti-SATB1 antibody - C-terminal (ab228772) at 1/5000 dilution + Mouse thymus tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 86 kDa7.5% SDS-PAGE gel.