Anti-Zhangfei antibody (ab28700)
Key features and details
- Rabbit polyclonal to Zhangfei
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Zhangfei antibody
See all Zhangfei primary antibodies -
Description
Rabbit polyclonal to Zhangfei -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Zhangfei.
Read Abcam's proprietary immunogen policy (Peptide available as ab28713.) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: 293T Embryonic Kidney cell line (Human), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), HeLa (Human epithelial carcinoma cell line), PC3 (Human prostate cancer cell line), NIH 3T3 (Mouse embryonic fibroblast cell line). This antibody also gave a positive signal in the following tissue lysates: Human Heart, Human Brain, Human Kidney, Human Liver.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).Target
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Function
Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells. -
Tissue specificity
In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver. -
Sequence similarities
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. Colocalizes at punctate nuclear structures with CREB3 and HCFC1. - Information by UniProt
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Database links
- Entrez Gene: 58487 Human
- Entrez Gene: 233490 Mouse
- Omim: 606444 Human
- SwissProt: Q9NS37 Human
- SwissProt: Q91ZR3 Mouse
- Unigene: 535319 Human
- Unigene: 714910 Human
- Unigene: 29496 Mouse
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Alternative names
- 1110034C16Rik antibody
- 6330417B10Rik antibody
- AI225749 antibody
see all
Images
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Western blot - Anti-Zhangfei antibody (ab28700)All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : PC-3 whole cell lysate (ab3954)
Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesIn the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.
Datasheets and documents
References (6)
ab28700 has been referenced in 6 publications.
- Chen F et al. Activation of CREBZF Increases Cell Apoptosis in Mouse Ovarian Granulosa Cells by Regulating the ERK1/2 and mTOR Signaling Pathways. Int J Mol Sci 19:N/A (2018). PubMed: 30413092
- Chen F et al. Construction and expression of lentiviral vectors encoding recombinant mouse CREBZF in NIH 3T3 cells. Plasmid 76C:24-31 (2014). PubMed: 25195838
- López-Mateo I et al. The transcription factor CREBZF is a novel positive regulator of p53. Cell Cycle 11:3887-95 (2012). PubMed: 22983008
- Misra J et al. Curcumin Differentially Regulates Endoplasmic Reticulum Stress through Transcriptional Corepressor SMILE (Small Heterodimer Partner-interacting Leucine Zipper Protein)-mediated Inhibition of CREBH (cAMP Responsive Element-binding Protein H). J Biol Chem 286:41972-84 (2011). WB . PubMed: 21994947
- Xie YB et al. Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation. J Biol Chem 284:28762-74 (2009). WB ; Human . PubMed: 19690166
- Xie YB et al. SMILE, a new orphan nuclear receptor SHP-interacting protein, regulates SHP-repressed estrogen receptor transactivation. Biochem J 416:463-73 (2008). WB ; Human . PubMed: 18657049
Images
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Western blot - Anti-Zhangfei antibody (ab28700)All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 9 : PC-3 whole cell lysate (ab3954)
Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesIn the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.
