Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: SATB1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: THP1 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab109122 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109122 was shown to specifically react with SATB1 in wild-type HAP1 cells as signal was lost in SATB1 knockout cells. Wild-type and SATB1 knockout samples were subjected to SDS-PAGE. Ab109122 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

Ab109122 staining SATB1 in Jurkat (Human T cell leukemia T lymphocyte) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor^® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on Jurkat cell line.

All lanes : Anti-SATB1 antibody [EPR3951] (ab109122) at 1/1000 dilution

Lane 1 : Jurkat cell lysates
Lane 2 : Fetal thymus lysates
Lane 3 : Mouse thymus lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 86 kDa

Ab109122 staining SATB1 in THP-1 (Human monocytic leukemia monocyte) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized in 0.1% TritonX-100. Samples were incubated with primary antibody 1:1000 dilution (2.1 μg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Alexa Fluor® 594 Anti-alpha Tubulin [DM1A] – Microtubule marker, ab195889 was used as a counterstain antibody (1:200, 2.5 μg/ml). DAPI was used as a counterstain antibody. Confocal image showing nuclear staining on THP-1 cell line. 

ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human breast carcinoma, by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab109122 staining SATB1 in mouse skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde, blocked with 10% goat serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 12 hours. A FITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

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ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human ovarian adenocarcinoma tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.