Anti-UTF1 antibody (ab105090)
Key features and details
- Rabbit polyclonal to UTF1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-UTF1 antibody
See all UTF1 primary antibodies -
Description
Rabbit polyclonal to UTF1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Cow, Human, Pig, Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human UTF1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab119167) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9; IOUD2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab105090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Target
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Function
Acts as a transcriptional coactivator of ATF2. -
Domain
The leucine-zipper domain is required for coactivation activity. When this domain is deleted, the protein is able to stimulate transcription from a number of gene promoters. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8433 Human
- Entrez Gene: 22286 Mouse
- Entrez Gene: 681901 Rat
- Omim: 604130 Human
- SwissProt: Q5T230 Human
- SwissProt: Q6J1H4 Mouse
- Unigene: 458406 Human
- Unigene: 10205 Mouse
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Form
UTF1 is a transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells. Upon activation by Oct-3/4, UTF1 may be responsible for the rapid proliferation and tumorigenic properties of embryonic stem cells. Sox2 is also part of the Oct-3/4-containing complex able to bind the regulatory region of UTF1. UTF1 acts as a transcriptional coactivator of ATF2 and it binds to the N-terminal region of ATF2. Associates with the TFIID complex through interaction with TBP. -
Alternative names
- hUTF 1 antibody
- hUTF1 antibody
- Undifferentiated embryonic cell transcription factor 1 antibody
see all
Images
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Western blot - Anti-UTF1 antibody (ab105090)All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The expression profile observed is consistent with what has been described in the literature (PMID:17785516). -
Immunocytochemistry/ Immunofluorescence - Anti-UTF1 antibody (ab105090)ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) mouse ES cells at 1µg/ml.
Protocols
Datasheets and documents
References (1)
ab105090 has been referenced in 1 publication.
- Bi Y et al. Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation. Cell Rep 30:3917-3931.e5 (2020). PubMed: 32187559
Images
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Western blot - Anti-UTF1 antibody (ab105090)All lanes : Anti-UTF1 antibody (ab105090) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The expression profile observed is consistent with what has been described in the literature (PMID:17785516). -
Immunocytochemistry/ Immunofluorescence - Anti-UTF1 antibody (ab105090)ICC/IF image of ab105090 stained mouse embryonic stem cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105090, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) mouse ES cells at 1µg/ml.
