Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-OTULIN antibody [EPR19841] (ab211328)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19841] to OTULIN
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-OTULIN antibody [EPR19841]
See all OTULIN primary antibodies
Description

Rabbit monoclonal [EPR19841] to OTULIN
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T, MCF7 and PC-3 cell lysates; Human brain, fetal heart and fetal spleen lysates. IP: HEK-293T whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19841
Isotype

IgG
Research areas

Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)

All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : OTULIN knockout HEK-293T whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 2 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)

All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

Lane 1 : Human brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time : Lane 1-3: 3 minutes; Lane 4-5: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)

OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: HEK-293T whole cell lysate 10 µg (Input).

Lane 2: ab211328 IP in HEK-293T whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211328 in HEK-293T whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-OTULIN antibody [EPR19841] (ab211328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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