Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OTULIN antibody [EPR19841] (ab211328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19841] to OTULIN
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-OTULIN antibody [EPR19841]
    See all OTULIN primary antibodies

  • Description

    Rabbit monoclonal [EPR19841] to OTULIN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, MCF7 and PC-3 cell lysates; Human brain, fetal heart and fetal spleen lysates. IP: HEK-293T whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)
    Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)
    All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : OTULIN knockout HEK-293T whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)
    Western blot - Anti-OTULIN antibody [EPR19841] (ab211328)
    All lanes : Anti-OTULIN antibody [EPR19841] (ab211328) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal spleen lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



     

    Exposure time : Lane 1-3: 3 minutes; Lane 4-5: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)
    Immunoprecipitation - Anti-OTULIN antibody [EPR19841] (ab211328)

    OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: HEK-293T whole cell lysate 10 µg (Input). 

    Lane 2: ab211328 IP in HEK-293T whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211328 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-OTULIN antibody [EPR19841] (ab211328)
    Anti-OTULIN antibody [EPR19841] (ab211328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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