Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
![Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)](https://www.abcam.com/ps/products/271/ab271992/Images/ab271992-1-ab211328285790antiotulinantibodyepr19841immunoprecipitation.jpg "Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19841] to OTULIN - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-OTULIN antibody [EPR19841] - BSA and Azide free
See all OTULIN primary antibodies -
Description
Rabbit monoclonal [EPR19841] to OTULIN - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, MCF7 and PC-3 cell lysates; Human brain, fetal heart and fetal spleen lysates. IP: HEK-293T whole cell lysate.
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General notes
ab271992 is the carrier-free version of ab211328. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19841 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
OTULIN was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate with ab211328 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211328 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: ab211328 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211328 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211328). -
![Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)](https://www.abcam.com/ps/products/271/ab271992/Images/ab271992-2-benefits-of-recombinant-antibodies.png)
Anti-OTULIN antibody [EPR19841] - BSA and Azide free (ab271992)
