Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1424] to Band 3/AE 1 (phospho Y21)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424]
See all Band 3/AE 1 primary antibodies -
Description
Rabbit monoclonal [EPR1424] to Band 3/AE 1 (phospho Y21) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human Band 3/AE 1. The exact sequence is proprietary.
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Positive control
- Human red blood cell lysates, Pervanadate treated.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Band 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1424 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (purified)
Lane 1 : Untreated RBC
Lane 2 : RBC treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (unpurified)
Lane 1 : Human red blood cell lysate, untreated
Lane 2 : Human red blood cell lysate, Pervanadate treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
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