Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [PMab-1] to Podoplanin / gp36 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free
    See all Podoplanin / gp36 primary antibodies

  • Description

    Rat monoclonal [PMab-1] to Podoplanin / gp36 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung tissue lysate. IHC-P: Mouse kidney and lung tissue. IHC-Fr: Mouse kidney and lung tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256564 is the carrier-free version of ab256559. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559) at 1/1000 dilution + mouse lung tissue lysate at 20 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 16 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time 26 secs.

    This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse lung is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the podocytes in the mouse kidney is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000  dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

  • Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
    Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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