Anti-Band 3/AE 1 antibody (ab78067)
Key features and details
- Rabbit polyclonal to Band 3/AE 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Band 3/AE 1 antibody
See all Band 3/AE 1 primary antibodies -
Description
Rabbit polyclonal to Band 3/AE 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Band 3/AE 1 (N terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- Human placenta tissue
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General notes
Previously labelled as Band 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody (ab78067)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Band 3/AE 1 with ab78067 at 10 µg/ml.
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Western blot - Anti-Band 3/AE 1 antibody (ab78067)All lanes : Anti-Band 3/AE 1 antibody (ab78067) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate - fetal normal tissue (ab29890)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
