Overlay histogram showing K562 cells stained with unpurified ab129024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

ab129024 staining Glycophorin A in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/2500). ab97051(1/500) HRP-conjugated goat anti-rabbit IgG(H&L) was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

ab129024, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human lung tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, at 1/100 dilution staining Glycophorin A in formalin fixed paraffin embedded Human spleen tissue by immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, showing positive staining in Thyroid gland erythrocytes tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, showing negative staining in Normal brain tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, showing positive staining in Normal colon erythrocytes tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, showing positive staining in Normal kidney erythrocytes tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ab129024, unpurified, showing positive staining in Normal placenta erythrocytes tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129024).