Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 16, 2021

Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9299] to alpha 1 Spectrin
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-alpha 1 Spectrin antibody [EPR9299]
See all alpha 1 Spectrin primary antibodies
Description

Rabbit monoclonal [EPR9299] to alpha 1 Spectrin
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human alpha 1 Spectrin. The exact sequence is proprietary.
Positive control
- K562 and TF-1 cell lysates; Human lung and stomach tissues
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9299
Isotype

IgG
Research areas

Western blot - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

All lanes : Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : TF-1 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 280 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling alpha 1 Spectrin with ab154811 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin embedded Human normal liver, erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin embedded Human placenta, erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin embedded Human normal spleen, erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Immunohistochemical analysis of paraffin embedded Human normal uterus, erythrocytes tissue using ab154811 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-alpha 1 Spectrin antibody [EPR9299] (ab154811)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com