Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85)
Suitable for: WB, ICC/IF, PepArr
Reacts with: Mouse, Human

Product name

Anti-Histone H2B (mono methyl K85) antibody [EPR17613]
See all Histone H2B primary antibodies
Description

Rabbit monoclonal [EPR17613] to Histone H2B (mono methyl K85)
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
PepArr	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse ovary lysate; HL-60, HeLa, NIH:OVCAR-3, MOLT-4, THP-1, K562, F9, RAW 264.7 and NIH/3T3 cell lysates. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17613
Isotype

IgG
Research areas

Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/500 dilution + Mouse ovary lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/5000 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

All lanes : Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781) at 1/1000 dilution

Lane 1 : Human ovary lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : NIH:OVCAR-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia cell line) cell lysate
Lane 5 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 6 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 7 : F9 (mouse embryonic testicular cancer cell line) cell lysate
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 9 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% BSA/TBST.

Under our testing conditions (using whole cell extract and BSA blocking) we detected non-specific bands in addition to the BOI.
Peptide Array - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

ab177781 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H2B (mono methyl K85) with ab177781 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/500 dilution (red).

The negative controls are as follows:

-ve control 1: ab177781 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/500 dilution.

-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution.
Anti-Histone H2B (mono methyl K85) antibody [EPR17613] (ab177781)

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