Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K79) - ChIP Grade, purified
- Suitable for: ICC/IF, ChIP-sequencing, WB, ChIP, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K79) - ChIP Grade, purified -
Host species
Rabbit -
Specificity
Also expected to react with Yeast. -
Tested applications
Suitable for: ICC/IF, ChIP-sequencing, WB, ChIP, Dot blotmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K79) conjugated to keyhole limpet haemocyanin.
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Positive control
- ICC/IF: Human osteosarcoma (U2OS) cells. ChIP: HeLaS3 cells. WB: Histone extracts from HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)
ChIP-seq analysis, labeling Histone H3 (tri methyl K79) with ab195500 at 2 µg. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The peak distribution is shown along the complete sequence and a 600 kb region of the X-chromosome (A and B) and in a 2 Mb region from chromosome 11 (C) and in a 100 kb region surrounding the GAPDH positive control gene (D).
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ChIP - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)ChIP analysis using HeLas3 cells, labeling Histone H3 (TRI methyl K79) with ab195500. ChIP was performed using sheared chromatin from 1000,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and and for exon 2 of the inactive myoglobin gene. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.
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Dot Blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)Dot Blot analysis of peptides containing modified and unmodified sequences of histone H3 and H4, labeling Histone H3 (tri methyl K79) with ab195500 at 1/20000 dilution. 0.2-100 pmol of the peptide containing the respective histone modification were spotted onto the membrane.
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Western blot - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500) at 1/1000 dilution (in TBS-Tween containing 5% skimmed milk) + histone extracts of HeLa cells at 15 µg
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (tri methyl K79) antibody - ChIP Grade, purified (ab195500)Immunofluorescent analysis of Human osteosarcoma (U2OS) cells labeling Histone H3 (tri methyl K79) with ab195500 at 1/300 dilution in blocking solution followed by row A: an anti-rabbit antibody conjugated to Alexa568 (left) or with DAPI (right), which specifically labels DNA. Rows B-C: staining of the cells with the ab195500 after incubation of the antibody with 2 ng/µl H4K20me3 and H3K79me3 peptide, respectively. Cells were fixed with 2.5% formaldehyde for 30 minutes and blocked with PBS/TX-100 containing 1% BSA.
