Human Myoglobin ELISA Kit (ab108652)
Key features and details
- Sensitivity: 5 ng/ml
- Range: 5 ng/ml - 1000 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Myoglobin ELISA Kit
See all Myoglobin kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
= 5 ng/ml -
Range
5 ng/ml - 1000 ng/ml -
Recovery
102.8 %
Sample specific recovery Sample type Average % Range Serum 102.8 39ng/ml - 780ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.
A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Anti-Myoglobin Coated Microplate (12 x 8 wells) 1 unit Anti-Myoglobin HRP Conjugate 1 x 22ml Myoglobin Standard 0 - 0 ng/mL 1 x 0.5ml Myoglobin Standard 1 - 25 ng/mL 1 x 0.5ml Myoglobin Standard 2 - 100 ng/mL 1 x 0.5ml Myoglobin Standard 3 - 250 ng/mL 1 x 0.5ml Myoglobin Standard 4 - 500 ng/mL 1 x 0.5ml Myoglobin Standard 5 - 1,000 ng/mL 1 x 0.5ml Sample Diluent 1 x 25ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml -
Research areas
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Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
Sequence similarities
Belongs to the globin family. - Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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Database links
- Entrez Gene: 4151 Human
- Omim: 160000 Human
- SwissProt: P02144 Human
- Unigene: 517586 Human
Images
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Typical Standard Curve
Representative standard curve using ab108652
