Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Thursday February 25, 2021

HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-SQSTM1 / p62 antibody [EPR4844]
See all SQSTM1 / p62 primary antibodies
Description

HRP Rabbit monoclonal [EPR4844] to SQSTM1 / p62
Host species

Rabbit
Conjugation

HRP
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF-7 and HeLa whole cell lysates. IHC: normal human spleen tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4844
Isotype

IgG
Research areas

Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/5000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SQSTM1 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 62 kDa

Exposure time: 12 minutes

ab194720 was shown to specifically react with SQSTM1 in wild-type HAP1 cells as signal was lost in SQSTM1 knockout cells. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab194720 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor^® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
Western blot - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

All lanes : HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa whole cell lysate (ab150035)

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 20 seconds

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194720 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

IHC image of SQSTM1 / p62 staining in a section of formalin-fixed paraffin-embedded normal human spleen tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194720 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
HRP Anti-SQSTM1 / p62 antibody [EPR4844] (ab194720)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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