Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Rel antibody [EPR2559(2)] (ab133251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2559(2)] to c-Rel
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-c-Rel antibody [EPR2559(2)]
    See all c-Rel primary antibodies

  • Description

    Rabbit monoclonal [EPR2559(2)] to c-Rel

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 and HeLa cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
    Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: c-Rel knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab133251 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab133251 was shown to recognize c-Rel when c-Rel knockout samples were used, along with additional cross-reactive bands. Wild-type and c-Rel knockout samples were subjected to SDS-PAGE. ab133251 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Anti-c-Rel antibody [EPR2559(2)] (ab133251)
    Anti-c-Rel antibody [EPR2559(2)] (ab133251)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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