Anti-c-Rel (phospho S503) antibody (ab30624)
.jpg "Anti-c-Rel (phospho S503) antibody (ab30624)")
Key features and details
- Rabbit polyclonal to c-Rel (phospho S503)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Rel (phospho S503) antibody
See all c-Rel primary antibodies -
Description
Rabbit polyclonal to c-Rel (phospho S503) -
Host species
Rabbit -
Specificity
ab30624 recognises endogenous levels of c-Rel only when phosphorylated at Serine 503. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human c-Rel around the phosphorylation site of Serine 503.
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Positive control
- Breast carcinoma, MDA-MB-435 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-Rel (phospho S503) antibody (ab30624)All lanes : Anti-c-Rel (phospho S503) antibody (ab30624) at 1/500 dilution
Lane 1 : extracts from
MDA-MB-435 cells (5-30ug).
Lane 2 : extracts from
MDA-MB-435 cells (5-30ug), preincubated with synthesized peptide (negative control).
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Rel (phospho S503) antibody (ab30624)Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 c-Rel (Phospho- Ser503) Antibody (ab30624). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
