Anti-UXT antibody (ab77483)
Key features and details
- Goat polyclonal to UXT
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UXT antibody
See all UXT primary antibodies -
Description
Goat polyclonal to UXT -
Host species
Goat -
Specificity
ab77483 is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
C-DHRDKVYEQLAKY
, from an internal region of Human UXT (NP_004173.1; NP_705582.1). -
Positive control
- Human Kidney and Skeletal Muscle lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77483 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UXT antibody (ab77483)
ab77483 (5µg/ml) staining of UXT in paraffin embedded Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of cystoplasm in all epithelial cells, but nuclear staining in some of them.
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Western blot - Anti-UXT antibody (ab77483)Anti-UXT antibody (ab77483) at 0.03 µg/ml + Human Kidney lysate (in RIPA buffer) at 35 µg
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
