Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

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Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5079(N)] to SIRT6
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-SIRT6 antibody [EPR5079(N)]
See all SIRT6 primary antibodies
Description

Rabbit monoclonal [EPR5079(N)] to SIRT6
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

within Human SIRT6 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8N6T7
Positive control
- WB: K562, Jurkat, 293T, HepG2 and HeLa whole cell lysate (ab150035).
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5079(N)
Isotype

IgG
Research areas

Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SIRT6 knockout HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 39 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Lanes 1-2: Merged signal (red and green). Green - ab176345 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.

ab176345 Anti-SIRT6 antibody [EPR5079(N)] was shown to specifically react with SIRT6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265054 (knockout cell lysate ab257673) was used. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. ab176345 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SIRT6 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 39 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab176345 (unpurified) observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab176345 was shown to specifically recognize SIRT6 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when SIRT6 knockout samples were examined. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. Ab176345 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution (Purified)

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 39 kDa
Observed band size: 42-43 kDa why is the actual band size different from the predicted?

The doublets detected and observed molecular weights are consistent with what have been described in literatures PMID: 30542728, 29143563, 24169447 and 30670969.
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

All lanes : Anti-SIRT6 antibody [EPR5079(N)] (ab176345) at 1/1000 dilution (unpurified)

Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 39 kDa
Anti-SIRT6 antibody [EPR5079(N)] (ab176345)

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