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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway

Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)

Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NFkB p100/NFKB2 antibody - C-terminal
    See all NFkB p100/NFKB2 primary antibodies
  • Description

    Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab227096 specifically recognizes the NFkB p100/NFKB2 isoform, but not the cleaved p52 isoform.

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human NFkB p100/NFKB2 (C terminal). The exact sequence is proprietary.
    Database link: Q00653

  • Positive control

    • WB: A431 cell extract; HeLa whole cell extract; HeLa, HepG2 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as NFkB p100

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : NFkB p100/NFKB2 Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 97 kDa

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)

    NFkB p100/NFKB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) with 2 µg ab227096. Western blot was performed from the immunoprecipitate using ab227096. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Jurkat whole cell lysate 30 μg.

    Lane 2: Control IP in Jurkat whole cell lysate using 2 μg of preimmune rabbit IgG.

    Lane 3: ab227096 IP in Jurkat whole cell lysate.

     

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution

    Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : NFkB p100/NFKB2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Western blot - Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
    Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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