Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096)
Key features and details
- Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p100/NFKB2 antibody - C-terminal
See all NFkB p100/NFKB2 primary antibodies -
Description
Rabbit polyclonal to NFkB p100/NFKB2 - C-terminal -
Host species
Rabbit -
Specificity
ab227096 specifically recognizes the NFkB p100/NFKB2 isoform, but not the cleaved p52 isoform.
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment within Human NFkB p100/NFKB2 (C terminal). The exact sequence is proprietary.
Database link: Q00653 -
Positive control
- WB: A431 cell extract; HeLa whole cell extract; HeLa, HepG2 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
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General notes
This product was previously labelled as NFkB p100
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : NFkB p100/NFKB2 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 97 kDa
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Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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NFkB p100/NFKB2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) with 2 µg ab227096. Western blot was performed from the immunoprecipitate using ab227096. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Jurkat whole cell lysate 30 μg.
Lane 2: Control IP in Jurkat whole cell lysate using 2 μg of preimmune rabbit IgG.
Lane 3: ab227096 IP in Jurkat whole cell lysate.
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/500 dilution
Lane 1 : Wild type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : NFkB p100/NFKB2 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 97 kDa7.5% SDS-PAGE gel.
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Anti-NFkB p100/NFKB2 antibody - C-terminal (ab227096) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 97 kDa7.5% SDS-PAGE gel.