Anti-NFkB p100/NFKB2 (phospho S865) antibody (ab31474)
Key features and details
- Rabbit polyclonal to NFkB p100/NFKB2 (phospho S865)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p100/NFKB2 (phospho S865) antibody
See all NFkB p100/NFKB2 primary antibodies -
Description
Rabbit polyclonal to NFkB p100/NFKB2 (phospho S865) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of NFkB p100/NFKB2 protein only when phosphorylated at Serine 865.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human NFkB p100/NFKB2 (phospho S865). The exact sequence is proprietary.
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Positive control
- WB: extracts of ovary cancer cells. IHC-P:breast carcinoma tissue.
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General notes
This product was previously labelled as NFkB p100
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 (phospho S865) antibody (ab31474)
Ab31474, at a dilution of 1/50, staining NFkB p100/NFKB2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: treated with ab31474.
Right image: treated with the same antibody preincubated with sythesized peptide. -
Western blot - Anti-NFkB p100/NFKB2 (phospho S865) antibody (ab31474)All lanes : Anti-NFkB p100/NFKB2 (phospho S865) antibody (ab31474) at 1/500 dilution
Lane 1 : Extracts of ovary cancer cells (5-30ug).
Lane 2 : Extracts of ovary cancer cells (5-30ug). Antibody pre-incubated with synthesized peptide.
Secondary
All lanes : goat-anti-rabbit IgG-AP-conjugate.
Predicted band size: 54 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 (phospho S865) antibody (ab31474)ICC/IF image of ab31474 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31474, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
