Anti-NFkB p100/NFKB2 antibody (ab209799)
Key features and details
- Rabbit polyclonal to NFkB p100/NFKB2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NFkB p100/NFKB2 antibody
See all NFkB p100/NFKB2 primary antibodies -
Description
Rabbit polyclonal to NFkB p100/NFKB2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human NFkB p100/NFKB2 aa 700 to the C-terminus.
Database link: Q00653-4 -
Positive control
- WB: A431, NIH/3T3, NBT-II cell lysates; IHC-P: Human appendix, lymph node, skeletal muscle, cerebral cortex, kidney, and testis tissues; ICC: A431 cells.
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General notes
This product was previously labelled as NFkB p100
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling NFkB p100/NFKB2 using ab209799 at 4 μg/ml (green).
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Western blot - Anti-NFkB p100/NFKB2 antibody (ab209799)All lanes : Anti-NFkB p100/NFKB2 antibody (ab209799) at 0.4 µg/ml
Lane 1 : A431 (human epidermoid carcinoma cell line) transfected with control siRNA, cell lysate
Lane 2 : A431 transfected with target specific siRNA probe #1, cell lysate
Lane 3 : A431 transfected with target specific siRNA probe #2, cell lysate
Predicted band size: 97 kDaLoading control: Anti-GAPDH
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Western blot - Anti-NFkB p100/NFKB2 antibody (ab209799)All lanes : Anti-NFkB p100/NFKB2 antibody (ab209799) at 1/250 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 97 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human appendix tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p100/NFKB2 antibody (ab209799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling p100/NFKB2 with ab209799 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
